Coding

Part:BBa_K368006:Design

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


CMV-Promoter + PhiC31o + SV40PA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2042
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 859
    Illegal AgeI site found at 1154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 201
    Illegal BsaI.rc site found at 538
    Illegal BsaI.rc site found at 781
    Illegal BsaI.rc site found at 941
    Illegal BsaI.rc site found at 957
    Illegal BsaI.rc site found at 1294
    Illegal SapI.rc site found at 444
    Illegal SapI.rc site found at 1356


Design Notes

-> "Jump-Or-Die"system


Source

PhiC31o: (Raymond CS et al: High-Efficiency FLP and PhiC31 Site-Specific Recombination in Mammalian Cells (2007)), plasmid bought from addgene CMV Promoter: should have been Biobrick, but we got something wrong...

References